报告题目：High speed imaging at and beyond the diffraction limit
报告人：Prof. Hari Shroff
邀请人：刘华锋
时间：2017年6月6日 10:00-11:30
地点：玉泉校区教三楼440室
Hari Shroff 是光学成像方面的专家，在PNAS，nature子刊等级别的期刊发表了近20篇高质量论文。
Dr. Hari Shroff received a B.S.E. in bioengineering from the University of Washington in 2001, and under the supervision of Dr. Jan Liphardt, completed his Ph.D. in biophysics at the University of California at Berkeley in 2006. He spent the next three years performing postdoctoral research under the mentorship of Eric Betzig at the Howard Hughes Medical Institute's Janelia Farm Research Campus where his research focused on development of photactivated localization microscopy (PALM), an optical superresolution technique. Dr. Hari Shroff is now chief of NIBIB's Section on High Resolution Optical Imaging laboratory, where he and his staff are developing new imaging tools for application in biological and clinical research. And now he is an adjunct professor at the College of Optical Science and Engineering.
题目：High speed imaging at and beyond the diffraction limit
In this report,Dr. Hari Shroff will present SIM implementations that enable resolution doubling in live samples > 10-20x thicker than possible with conventional SIM, as well as hardware modifications that enable effectively ‘instant’ SIM imaging at rates 10-100x faster than other SIM. New applications of instant SIM, including combination with total internal reflection (TIRF) and with adaptive optics will also be discussed. The second half of the talk will focus on the development of inverted selective plane illumination microscopy (iSPIM), and subsequent application to the noninvasive study of neurodevelopment in nematodes. Dr. Hari Shroff will discuss progress that quadruples the axial resolution of iSPIM by using a second specimen view, enabling imaging with isotropic spatial resolution (dual-view iSPIM, or diSPIM). Newer multiview microscopes with more objectives and more views, further improving spatial resolution, will also be described. Applications of these technologies will be presented, including computational methods for untwisting worm embryos and calcium imaging in freely moving embryos.
