[Optics Express]Strategies to maximize the performance of a STED microscope

篇名

Strategies to maximize the performance of a STED microscope

刊名

Optics Express

作者

Silvia Galiani, Benjamin Harke, Giuseppe Vicidomini et al.

DOI

10.1364/OE.20.007362

日期

2012-3-26

摘要

 In stimulated emission depletion (STED) microscopy, the spatial

resolution scales as the inverse square root of the STED beam's intensity. However, to fully exploit the maximum effective resolution achievable for a given STED beam’s intensity, several experimental precautions have to be considered. We focus our attention on the temporal alignment between the excitation and STED pulses and the polarization state of the STED beam.We present a simple theoretical framework that help to explain their influence on the performance of a STED microscope and we validate the results by imaging calibration and biological samples with a custom made STED architecture based on a supercontinuum laser source. We alsohighlight the advantages of using time gating detection in terms of temporal alignment.

推荐理由

1. 从理论和实验两个方面，首次提出了聚焦光斑对于最终系统成像质量影响的量化评价。

2. 首次对于基于超连续光源STED体系的清晰表述。

供稿人

王会会