Research Highlights

Professor Qian Jun and Professor Gao Lixia of Zhejiang University and Academician Tang Benzhong of HKUST develop biologically excretable Aggregation‐Induced Emission Dots for Visualizing Through the Marmosets Intravitally


Superb reliability and biocompatibility equip aggregationinduced emission (AIE) dots with tremendous potential for fluorescence bioimaging. However, there is still a chronic lack of design instructions of excretable and bright AIE emitters. Here, a kind of PEGylated AIE (OTPABBT) dots with strong absorption and extremely high second nearinfrared region (NIRII) PLQY of 13.6% is designed, and a longaliphaticchain design blueprint contributing to their excretion from an animal's body is proposed. Assisted by the OTPABBT dots with bright fluorescence beyond 1100 nm and even 1500 nm (NIRIIb), largedepth cerebral vasculature (beyond 600 µm) as well as realtime blood flow are monitored through a thinned skull, and noninvasive NIRIIb imaging with rich highspatialfrequency information gives a precise presentation of gastrointestinal tract in marmosets. Importantly, after intravenous or oral administration, the definite excretion of OTPABBT dots from the body is demonstrated, which provides influential evidence of biosafety.

NIR‐II fluorescence microscopy image of brain vasculature.

NIR‐IIb fluorescence noninvasive gastrointestinal imaging with high spatial‐frequency.

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